Science Robotics

The PDF file includes:

• Supplementary Methods
• Fig. S1. Characterization of Fe₃O₄ NPs.
• Fig. S2. TEM image of nanogels.
• Fig. S3. Dependence of PTX encapsulated in each milligram nanogels on PTX loading amount.
• Fig. S4. CLSM images of EM@nanogels.
• Fig. S5. Movement of neutrophils incubated in different suspensions.
• Fig. S6. Morphological image of neutrophils.
• Fig. S7. CLSM images of neutrophils, neutrobots, neutrophils incubated with nanogels to show cell viability.
• Fig. S8. RMF navigation system.
• Fig. S9. Velocity of neutrobots (nanogels inside) under RMF with different strength and frequency.
• Fig. S10. Motion of neutrobots on the substrate and suspended in liquid under RMF (15 mT, 2 Hz).
• Fig. S11. Motion of neutrobots under gradient MF (~800 mT).
• Fig. S12. Photograph of the model blood flow system using a fresh blood–filled microfluidic.
• Fig. S13. Frequency range of neutrobot swarm formation under RMF (18 mT).
• Fig. S14. Simulation of neutrobots’ positions in a tetramer swarm in Y and Z axis change with time.
• Fig. S15. Schematic layout of Ibidi μ-Slide Chemotaxis^3D.
• Fig. S16. Scheme illustration of measurement of TAD.
• Fig. S17. Chemotactic motion of neutrophils in CG.
• Fig. S18. Velocity of neutrobots on the surface of blood vessel and PDMS substrate under RMF (15 mT, 2 Hz).
• Fig. S19. Photograph of actual Transwell setup in 24-well plate.
• Fig. S20. Schematic and microscope images of neutrophils and neutrobots going through model BBB.
• Fig. S21. CLSM images of EM@nanogel-loaded neutrobots before treated with fMLP or PMA.
• Fig. S22. CLSM images show neutrobots deliver EM@nanogels to G422 cells.
• Fig. S23. CLSM image of the brain-frozen section of brain harvest from glioma-bearing mouse.
• Fig. S24. Helmholtz coil magnetic field device used in animal experiment.
• Fig. S25. Targeting ratio of neutrobots to main organs of glioma-bearing mice after different treatment.
• Fig. S26. T₂-weighted MRI of blood, neutrobots, and magnetic NPs with different concentration.
• Fig. S27. Ultrathin-section TEM images of glioma to show neutrobots inside glioma tissue.
• Fig. S28. Statistics of neutrobots in the histosection of glioma.
• Fig. S29. Change in the body weight of glioma-bearing mice after different treatment.
• Fig. S30. Histological observation of main organs collected from glioma-bearing mice after different treatment.
• Reference (59)

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mp4 format). Schematic illustration for synthesis and dual-responsive active delivery of neutrobots.
• Movie S2 (.mp4 format). Motion of multiple neutrobots moving toward a certain direction under RMF (15 mT, 2 Hz).
• Movie S3 (.mp4 format). Motion of neutrobots on the substrate (blue trajectory) and suspended in liquid (green trajectory) under RMF (15 mT, 2 Hz).
• Movie S4 (.mp4 format). Motion of neutrobots with a wave-like trajectory and star-like trajectory under RMF (15 mT, 2 Hz).
• Movie S5 (.mp4 format). Motion of neutrobots under gradient MF (~800 mT).
• Movie S6 (.mp4 format). Motion of neutrobots against flow under RMF.
• Movie S7 (.mp4 format). Formation of chain from mono-neutrobot to tetra-neutrobots under RMF (18 mT, 15 Hz).
• Movie S8 (.mp4 format). Magnetically powered movement of tetra-neutrobot swarm chain under RMF (18 mT, 15 Hz).
• Movie S9 (.mp4 format). Chemotactic motion of neutrobots along CG.
• Movie S10 (.mp4 format). Dual-responded motion of neutrobots on the blood vessel wall.
• Movie S11 (.mp4 format). Neutrobots moving across the BBB model by chemotactic motion.