Science Robotics

Supplementary Materials

The PDF file includes:

  • Fig. S1. The velocities of microrobots under external magnetic fields with various rotating frequencies.
  • Fig. S2. Manipulation of the helical microrobot in a horizontal microfluidic channel and the spherical microrobot in a vertical microfluidic channel.
  • Fig. S3. Three-dimensional images of hippocampal NSCs distributed on helical and spherical microrobots.
  • Fig. S4. The number of proliferated stem cells on the microrobots and the numbers of cells on the microrobots after differentiation into astrocytes, oligodendrocytes, and neurons.
  • Fig. S5. Targeted HCT116 cell delivery and transplantation using a microrobot in BoC.
  • Fig. S6. Fluorescence imaging of the cells attached to the microrobot, ATP content in the cells for quantitative analysis of the cell viability of the treatment group, and reconstructed confocal image of the immunofluorescence staining of the HCT116 cells attached to the helical microrobot.
  • Fig. S7. The process of hanging drop cell culture with a spherical microrobot.
  • Fig. S8. Measured magnetic field intensity and gradient used for the in vivo experiment.
  • Table S1. Design parameters for each microrobot.
  • Legends for movies S1 to S8
  • References (5574)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Magnetic manipulation of the helical and spherical microrobots using a rotating magnetic field.
  • Movie S2 (.mp4 format). Three-dimensional animation of the confocal microscopic images of the differentiation of astrocytes, oligodendrocytes, and neurons on the helical and spherical microrobots.
  • Movie S3 (.mp4 format). Magnetic manipulation of the helical and spherical microrobots after hippocampal NSC attachment.
  • Movie S4 (.mp4 format). Targeted cell delivery in in vitro microorgan network using a magnetically actuated microrobot.
  • Movie S5 (.mp4 format). Microtissue cultivation after targeted cell delivery using a microrobot in the BoC.
  • Movie S6 (.mp4 format). Manipulation of the helical and spherical microrobots in a rat brain blood vessel for an ex vivo test.
  • Movie S7 (.mp4 format). Manipulation of the helical and spherical microrobots in a ventricle of a sagittal brain slice to mimic an in vivo environment.
  • Movie S8 (.mp4 format). Magnetic manipulation of the spherical microrobot with attached hTMSCs.

Files in this Data Supplement: