Supplementary Material for:

Soft erythrocyte-based bacterial microswimmers for cargo delivery

Yunus Alapan, Oncay Yasa, Oliver Schauer, Joshua Giltinan, Ahmet F. Tabak, Victor Sourjik, Metin Sitti*

*Corresponding author. Email: sitti{at}is.mpg.de

Published 25 April 2018, Sci. Robot. 3, eaar4423 (2018)
DOI: 10.1126/scirobotics.aar4423

This PDF file includes:

• Fig. S1. Bioengineered E. coli MG1655 for biotin attachment peptide expression of the cell membrane.
• Fig. S2. Characterization of TER-119 antigen presence on mouse RBCs and binding of biotin-conjugated TER-119 antibodies for streptavidin modification.
• Fig. S3. Bioengineered E. coli MG1655 attachment to DOX-loaded RBCs.
• Fig. S4. Fluorescence microscopy characterization of DOX loading into RBCs.
• Fig. S5. Flow cytometry density plots for RBCs without hypotonic isotonic treatment.
• Fig. S6. Flow cytometry population selection for RBCs.
• Fig. S7. SPION-loaded RBCs are attracted to a permanent magnet.
• Fig. S8. Characterization of the loading of citric acid–coated SPIONs into RBCs using EDS.
• Fig. S9. Photo of the custom five electromagnetic coils mounted to an inverted optical microscope and used for the magnetic guidance of RBC microswimmers.
• Fig. S10. FITC-labeled RBC microswimmers are shown at the entrance of a microchannel.
• Fig. S11. Deformation of FITC-labeled RBC microswimmers inside a microchannel with the attached bacterium.
• Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mp4 format). Bacteria-driven RBC microswimmers.
• Movie S2 (.mp4 format). Magnetic steering of bacteria-driven RBC microswimmers.
• Movie S3 (.mp4 format). Passive deformation of RBC microswimmers in microchannels.
• Movie S4 (.mp4 format). Active deformation of an RBC cargo propelled by single bacterium.