Science Robotics

Supplementary Materials

Supplementary Material for:

Additive manufacturing of hydrogel-based materials for next-generation implantable medical devices

Sau Yin Chin,* Yukkee Cheung Poh, Anne-Céline Kohler, Jocelyn T. Compton, Lauren L. Hsu, Kathryn M. Lau, Sohyun Kim, Benjamin W. Lee, Francis Y. Lee, Samuel K. Sia*

*Corresponding author. Email: ss2735{at} (S.K.S.); sc2983{at} (S.Y.C.)

Published 4 January 2017, Sci. Robot. 2, eaah6451 (2017)
DOI: 10.1126/scirobotics.aah6451

This PDF file includes:

  • Methods
  • Fig. S1. Setup for microfluidics-based lithography with precise height control.
  • Fig. S2. Actuator for spinning hydrogel gears.
  • Fig. S3. COMSOL magnetic field simulation of the magnetic force acting on the driving gear.
  • Fig. S4. Gate valve design constructed out of PEG-based hydrogel.
  • Fig. S5. Microgear pump constructed using our fabrication strategy.
  • Fig. S6. A hydrogel microgear pump actuating inside a mouse.
  • Fig. S7. Fabrication of dextran-filled hydrogel “boxes” for in vitro diffusion assays.
  • Fig. S8. In vitro release of dextran encapsulated in PEGDA constructs.
  • Fig. S9. Graphs for early-time approximation of effective diffusion coefficient, Deff.
  • Fig. S10. Graphs for late-time approximation of effective diffusion coefficient, Deff.
  • Fig. S11. FRAP data.
  • Fig. S12. Dosing schedule for each mouse and the fluorescent signals detected at the end of each experiment.
  • Fig. S13. Linear movement design.
  • Fig. S14. Single rotating gears.
  • Fig. S15. Two-gear designs.
  • Fig. S16. Geneva drive design.
  • Fig. S17. Young’s moduli for PEG-400 gels with increasing percentage of 400-Da PEGDA (w/v) used in the prepolymer.
  • Fig. S18. Young’s moduli of polymerized PEGDA hydrogels with various PEG chain lengths.
  • Fig. S19. Schematic diagram of the Geneva drive device.
  • Fig. S20. In vitro release of different payloads from the Geneva drive device.
  • Fig. S21. Fabrication and complete assembly of a Geneva drive hydrogel MEMS device.
  • Fig. S22. Schematic diagram of the single-gear device.
  • Fig. S23. Subcutaneously implanted single-gear device in a tumor-bearing mouse.
  • Fig. S24. Explanted hydrogel MEMS device and histology of the surrounding tissue.
  • Fig. S25. Histology of bone tumor tissues.
  • Table S1. Gel compositions used in this study.
  • Table S2. Breakdown of the total time for constructing the Geneva drive device.
  • Table S3. Diffusion coefficients obtained from both FRAP experiments (DFRAP) and in vitro release of dextran from fabricated hydrogel constructs (Deff).
  • Table S4. Doxorubicin dosing schedule for different treatment groups.
  • Table S5. Device criteria and corresponding design considerations.
  • Legends for movies S1 to S3
  • References (6971)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Actuation of a linear gated manifold device.
  • Movie S2 (.mov format). Operation of locking mechanism.
  • Movie S3 (.mp4 format). Rotation of the hydrogel-based Geneva drive gear.

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